aav serotype 6 (aav6) particles Search Results


aav serotype 6 aav6 production  (Virovek Inc)
86
Virovek Inc aav serotype 6 aav6 production
Aav Serotype 6 Aav6 Production, supplied by Virovek Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav+serotype+6+%28aav6%29+particles/us12398182-1230-0-10?v=Virovek+Inc
Average 86 stars, based on 1 article reviews
aav serotype 6 aav6 production - by Bioz Stars, 2026-08
86/100 stars
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raav6 virus  (PackGene Biotech lnc)
95
PackGene Biotech lnc raav6 virus
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Raav6 Virus, supplied by PackGene Biotech lnc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav+serotype+6+%28aav6%29+particles/pmc10850376-39-1-6?v=PackGene+Biotech+lnc
Average 95 stars, based on 1 article reviews
raav6 virus - by Bioz Stars, 2026-08
95/100 stars
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aav serotype 6 (aav6) particles  (SignaGen)
90
SignaGen aav serotype 6 (aav6) particles
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav Serotype 6 (Aav6) Particles, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav+serotype+6+%28aav6%29+particles/bio_rxiv__2025__06__06__658308-142-0-10?v=SignaGen
Average 90 stars, based on 1 article reviews
aav serotype 6 (aav6) particles - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
adeno associated virus serotype 6 aav6 vectors encoding ns1  (Genechem)
86
Genechem adeno associated virus serotype 6 aav6 vectors encoding ns1
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Adeno Associated Virus Serotype 6 Aav6 Vectors Encoding Ns1, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav+serotype+6+%28aav6%29+particles/pm42235715-88-0-26?v=Genechem
Average 86 stars, based on 1 article reviews
adeno associated virus serotype 6 aav6 vectors encoding ns1 - by Bioz Stars, 2026-08
86/100 stars
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Image Search Results


Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the rAAV6 homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.

Journal: Frontiers in Cell and Developmental Biology

Article Title: In situ correction of various β-thalassemia mutations in human hematopoietic stem cells

doi: 10.3389/fcell.2023.1276890

Figure Lengend Snippet: Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the rAAV6 homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.

Article Snippet: The rAAV6 virus was purchased from PackGene, and the titer value was determined to be 1E+13 GC/mL by quantitative PCR detection.

Techniques: Derivative Assay, Selection, Marker, Flow Cytometry, Expressing